[Research progress of rapid detection of ochratoxin A and its application prospect in traditional Chinese medicine].

Ochratoxin A (OTA) is a toxic secondary metabolite mainly produced by Aspergillus and Penicillium species, with strong renal toxicity, teratogenic, carcinogenic, mutagenic effect. Studies have shown that OTA is not only widely contaminated in food and feed crops, but also has been widely contaminated in Chinese herbal medicines such as spices, licorice and so on. In view of OTA's universality and harmfulness, this paper summarizes the flow visualization test strip, microsphere, electrochemical sensor, surface enhanced Raman spectroscopy technology in OTA rapid detection, which provides reference for the research and application of high throughout detection instrument miniaturization in order to achieve OTA quick detection and simple operation.

[Application of natural plant pigments in enlarged health industry].

Natural plant pigment is rich in resources, with the features of natural color and environment friendly, which has a broad space for development and market prospects. In order to further develop and utilize of natural plant pigment, this paper mainly introduces the natural plant dyes in the domains of food, cosmetics and health care products on the historical development process and their application. In addition,this paper summarizes the application of representative natural plant pigment, dyes, and prospects the market of natural plant pigment, so as to provide reference for the development of natural plant pigment in the enlarged health industry of China.

Potential model for differential diagnosis between Crohn's disease and primary intestinal lymphoma.

AIM: To evaluate the usefulness of different parameters to differentiate Crohn's disease (CD) from primary intestinal lymphoma (PIL). METHODS: The medical records of 85 patients with CD and 56 patients with PIL were reviewed retrospectively. Demographic, clinical, laboratory, endoscopic, and computed tomographic enterography (CTE) parameters were collected. The univariate value of each parameter was analyzed. A differentiation model was established by pooling all the valuable parameters. Diagnostic efficacy was analyzed, and a receiver operating characteristic (ROC) curve was plotted. RESULTS: The demographic and clinical parameters that showed significant values for differentiating CD from PIL included age of onset, symptom duration, presence of diarrhea, abdominal mass, and perianal lesions (P < 0.05). Elevated lactate dehydrogenase and serum ß2-microglobulin levels suggested a PIL diagnosis (P < 0.05). The endoscopic parameters that showed significant values for differentiating CD from PIL included multiple-site lesions, longitudinal ulcer, irregular ulcer, and intraluminal proliferative mass (P < 0.05). The CTE parameters that were useful in the identification of the two conditions included involvement of ≤ 3 segments, circular thickening of the bowel wall, wall thickness > 8 mm, aneurysmal dilation, stricture with proximal dilation, "comb sign", mass showing the "sandwich sign", and intussusceptions (P < 0.05). The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of the differentiation model were 91.8%, 96.4%, 93.6%, 97.5%, and 88.5%, respectively. The cutoff value was 0.5. The area under the ROC curve was 0.989. CONCLUSION: The differentiation model that integrated the various parameters together may yield a high diagnostic efficacy in the differential diagnosis between CD and PIL.

[The expression of CD73 in CD4+ regulatory T cells in patients with new-onset systemic lupus erythematosus].

OBJECTIVE: To investigate the level of CD(73) expression in CD(4)(+) regulatory T (Treg) cells in patients with systemic lupus erythematosus (SLE) and explore its role in the pathogenesis of SLE. METHODS: We selected 29 untreated/active SLE patients and 22 healthy controls. Frequencies of CD(4)(+)CD(25)(+) CD(73)(+) T cells and levels of FOXP3 protein expressed in CD(4)(+)CD(73)(+), CD(4)(+)CD(25)(hi), CD(4)(+)CD(25)(+) T cells were analyzed by flow cytometry. Meanwhile, the levels of SLE disease activity index (SLEDAI), C reactive protein (CRP), ESR, immunoglobulin and complement were measured. RESULTS: The percent of CD(4)(+)CD(25)(+) CD(73)(+) T cells was decreased in new-onset SLE compared with healthy controls [(1.25 ± 1.32)% vs (2.35 ± 1.09)%, P < 0.01], and it had no correlation with the levels of SLEDAI, CRP, ESR, et al and anti-C(1q) and anti-nucleosome antibodies (P > 0.05 for each). Both in groups of new-onset SLE and healthy controls, CD(73) level expressed in CD(4)(+)CD(25)(hi) T cells [(29.05 ± 12.53)%, (43.35 ± 10.09)%] was higher than that expressed in CD(4)(+)CD(25)(+) T cells [(17.48 ± 6.92)%, (29.98 ± 10.39)%, P < 0.01]. In both SLE patients and healthy controls, levels of FOXP3 protein expressed in CD(4)(+)CD(73)(+) T cells [(65.36 ± 14.40)%, (63.80 ± 14.05)%] and CD(4)(+)CD(25)(hi) T cells [(67.30 ± 13.04)%, (56.30 ± 9.21)%] were higher than those in CD(4)(+)CD(25)(+) T cells [(45.70 ± 12.74)%, (43.98 ± 5.17)%, P < 0.001], while it had no significant difference between the CD(4)(+)CD(25)(hi) and CD(4)(+)CD(73)(+) T cells (P > 0.05). CONCLUSION: These results demonstrate that CD(73) may be a new surface marker of regulatory T cells, and the abnormal expression of CD(73) in Treg cells may participate in the pathogenesis of SLE.